Computational protocol: A Rationally Designed TNF-α Epitope-Scaffold Immunogen Induces Sustained Antibody Response and Alleviates Collagen-Induced Arthritis in Mice

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Protocol publication

[…] The tertiary structure of DTNF proteins were modeled in Modeller9V7 based on the X-ray crystal structure of DT and the mouse TNF-α mentioned above, and validated by Procheck []. MD simulations were performed as previously described using Desmond 3.0 in default settings and lasted for 300 ns []. The protein structures were visualized using pyMOL 0.99 software. […]

Pipeline specifications

Software tools PROCHECK, PyMOL
Applications Small-angle scattering, Protein structure analysis
Organisms Mus musculus