Dataset features

Specifications


Application: RNA-seq analysis, sRNA-seq analysis
Number of samples: 33
Release date: May 1 2014
Last update date: Oct 10 2018
Access: Public
Dataset link Assessing the ceRNA hypothesis with quantitative measurements of miRNA and target abundance

Experimental Protocol


Seventeen mRNA profiles were generated of 1) primary hepatocytes of mice expressing variable levels of a recombinant Adenovirus expressing the transcript of AldolaseA (Ad-AldoA), containing either 1 or 3 sites matching miR-122 or a mutated miR-122 site (no site), 2) primary hepatocytes derived from mice treated with Antagomir-122 (treatment group) or Antagomir-122mm (control group), 3) livers originating of a genetic model (Ldlr deficient mice) causing severe pathological changes in cholesterol metabolism, 4) livers of mice perfused with Insulin or PBS, and 5) livers of mice fed a high-fat or chow diet; most samples were sequenced in duplicate or triplicate by an Illumina HiSeq 2000. One small RNA profile was also generated from livers of mice fed a chow diet by Solexa sequencing.

Repositories


GEO

GSE52801

ArrayExpress

E-GEOD-52801

ENA

SRP033367

BioProject

PRJNA230067

Download


Contact


Vikram Agarwal