|Application:||SNP array data analysis, Gene expression microarray analysis|
|Number of samples:||14|
|Release date:||Sep 19 2009|
|Last update date:||Feb 22 2018|
|Diseases:||Dental Caries, Stomach Neoplasms, Tumor Lysis Syndrome|
|Dataset link||NMD inhibition fails to identify tumour suppressor genes in microsatellite stable gastric cancer cell lines|
Two non-commercial gastric cancer cell lines, GP202 and IPA220, were transfected with siRNA directed against UPF1, to specifically inhibit the nonsense mediated decay (NMD) pathway, and with siRNA directed against non-specific siRNA duplexes (CVII) as a control. Microarray expression experiments were performed in triplicate on 4x44 K agilent arrays by hybridizing RNA from UPF1-transfected cells against non-specific CVII-transfected cells. In addition, array CGH of the two cell lines was performed on 4x44K agilent arrays to obtain the DNA copy number profiles. Mutation analysis of GINI candidates was performed by sequencing.
BMC Med Genomics