Computational protocol: Developmental expression of membrane type 4-matrix metalloproteinase (Mt4-mmp/Mmp17) in the mouse embryo

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Protocol publication

[…] Statistical differences in protein expression and mRNA levels among different tissues and embryonic stages were assessed by one-way ANOVA followed by post-hoc Student-Newman-Keuls (SNK) multiple comparison test and Student’s t test. Analysis was performed using Graphpad Prism 6.0 and IBM SPSS Statistics v21. Significance was assigned at p<0.05. For Western blot analysis, relative intensity of the bands were quantified by Image J software (https://imagej.nih.gov/ij/) and normalized to α-tubulin ones. […]

Pipeline specifications

Software tools SPSS, ImageJ
Applications Miscellaneous, Microscopic phenotype analysis
Organisms Mus musculus
Diseases Neoplasms
Chemicals Glycosylphosphatidylinositols