Computational protocol: Decreased antimony uptake and overexpression of genes of thiol metabolism are associated with drug resistance in a canine isolate of Leishmania infantum

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Protocol publication

[…] To determine parasite susceptibility to SbIII and other anti-leishmania drugs such as AmB, paromomycin and miltefosine, 2 × 105 promastigotes were incubated in 96-well plates (100 μL) at 28 °C for 72 h in the presence of increasing drug concentrations before determining cell proliferation by the MTT colorimetric assay as described previously (). The 50% effective concentration (EC50) was defined as the drug concentration required for half maximal inhibition of cellular growth rate. The EC50 for each line was calculated by nonlinear regression analysis using SigmaPlot 2000 software for Windows (SPSS Inc., Chicago, IL, USA). Resistance index (RI) was calculated by dividing the EC50 obtained for the resistant line by the EC50 obtained for the parental susceptible line. […]

Pipeline specifications

Software tools SigmaPlot, SPSS
Application Miscellaneous
Organisms Canis lupus familiaris, Homo sapiens
Diseases Gastrointestinal Diseases, Infection, Leishmaniasis, Visceral, Zoonoses
Chemicals Amphotericin B, Sulfhydryl Compounds