Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 130
Release date: Mar 31 2017
Last update date: Apr 1 2017
Access: Public
Taxon: Mustela putorius furo, Sus scrofa, Homo sapiens
Dataset link Host responses that correlate with respiratory droplet transmission of swine-origin influenza viruses in ferrets.

Experimental Protocol


Ferrets were mock inoculated with 0.5mL media or inoculated with 105 TCID50 of the A/CA/04/09 (H1N1) or A/Sw/HK/ 210/10 (H1N1) influenza viruses intra-nasally. There are a total of 9 ferrets per virus group and six ferrets for the mock group. Ferrets were euthanized at day 1 (n = 4 per virus group) and day 2 (n = 5 per virus group) post-inoculation (p.i.). Three mock inoculated ferrets were euthanized at days 1 and 2 p.i. Tissues from nasal turbinate, soft palate, pharynx, trachea, bronchus, and lungs were collected at necropsy, placed in RNAlater overnight at 4°C and conserved at -80°C. RNA was extracted and gene expression was analyzed by custom 4x180K ferret-specific oligonucleotide arrays (Agilent Cat# G4862A, design ID 48472)

Repositories


GEO

GSE79282

BioProject

PRJNA315396

Download


Contact


Michael Katze