Dataset features

Specifications


Application: ChIP-seq analysis
Number of samples: 18
Release date: Jul 24 2013
Last update date: Oct 16 2017
Access: Public
Chemicals: Hydrogen Peroxide, Sirolimus
Dataset link The yeast Snt2 protein helps coordinate the transcriptional response to hydrogen-peroxide mediated oxidative stress (H2O2)

Experimental Protocol


Crosslinking ChIP analysis to identify sites of Snt2 or Ecm5 genomic localization before, 0.5 hours after, or 4 hours after treatment with H2O2 (final concentration 0.4 mM). Snt2 and Ecm5 were genomically tagged with a 13Myc tag at their C termini. ChIPs were performed using a Myc antibody on either Snt2-Myc or Ecm5-Myc strains, or on an untagged wildtype strain (BY4741) as a control. Inputs and ChIPs from untagged strain were sequenced as controls.

Repositories


GEO

GSE42971

ArrayExpress

E-GEOD-42971

ENA

SRP017602

BioProject

PRJNA184039

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