|Application:||Gene expression microarray analysis|
|Number of samples:||36|
|Release date:||Jan 1 2009|
|Last update date:||Jan 18 2013|
|Dataset link||T7-based linear amplification of low concentration mRNA samples using beads and microfluidics|
In this study we examined the IVT reaction in a microfluidic system apart from the other steps in the linear amplification process. Stock concentrations of cDNA were generated and known concentrations (down to single-cell equivalents) were packed onto the microfluidic or utilized as a benchtop process. IVT reactions were performed and efficiencies compared using microarray platforms.