Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 36
Release date: Jan 1 2009
Last update date: Jan 18 2013
Access: Public
Taxon: Homo sapiens
Dataset link T7-based linear amplification of low concentration mRNA samples using beads and microfluidics

Experimental Protocol


In this study we examined the IVT reaction in a microfluidic system apart from the other steps in the linear amplification process. Stock concentrations of cDNA were generated and known concentrations (down to single-cell equivalents) were packed onto the microfluidic or utilized as a benchtop process. IVT reactions were performed and efficiencies compared using microarray platforms.

Repositories


GEO

GSE13595

BioProject

PRJNA110707

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