Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 20
Release date: Sep 17 2018
Last update date: Oct 29 2018
Access: Public
Dataset link Transient expression of T-bet epigenetically imprints the Ifng locus for its expression in a distinct Tfh cell subset

Experimental Protocol


RNA-Seq analysis of gene expression in splenic CD4+CD44highCD25- T cells from naive mice that are either expressing T-bet or have a history of T-bet expression. RNA-Seq analysis of gene expression in antigen (AS15)-specific Tfh and non-Tfh cell subsets that were separated based on their history of T-bet expression. scDNase-seq analysis of antigen (AS15)-specific Tfh and non-Tfh cell subsets that were separated based on their history of T-bet expression. AS15-specific cells from draining lymph nodes of AS-15/CFA-immunized mice on day 16 were identified by tetramer staining. All the cell subsets were harvested by cell sorting after staining.

Repositories


GEO

GSE102959

ENA

SRP115960

BioProject

PRJNA399539

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