Dataset features

Specifications


Dataset type: Expression profiling by RT-PCR, Transcriptome or Gene expression
Number of samples: 15
Release date: Jan 24 2017
Last update date: Jan 31 2017
Access: Public
Diseases: Cataract, Neurodegenerative Diseases
Dataset link Identification of Circulating Fibrocytes and Dendritic Derivatives in Corneal Endothelium of Patients with Fuchs' Dystrophy [RT-qPCR array CAPH10410]

Experimental Protocol


This study was performed on CE monolayers with Descemet membrane from patients with late-onset FECD. Microarray expression analysis was performed on 8 samples (n = 4 FECD, n = 4 normal). RT-qPCR was done for 179 genes of interest and at least three reference genes (n = 9 FECD, n = 8 normal). Molecules of interest were validated with immunohistochemical and immunofluorescent staining on fresh corneal endothelial whole-mounts (n = 55 FECD, n = 15 normal). All stainings were optimized on external control tissues, which were included as batch controls. Dye swaps ensured specificity of double staining.

Repositories


GEO

GSE75675

BioProject

PRJNA305033

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Contact


Joost van den Oord