|Dataset type:||Expression profiling by RT-PCR, Transcriptome or Gene expression|
|Number of samples:||15|
|Release date:||Jan 24 2017|
|Last update date:||Jan 31 2017|
|Diseases:||Cataract, Neurodegenerative Diseases|
|Dataset link||Identification of Circulating Fibrocytes and Dendritic Derivatives in Corneal Endothelium of Patients with Fuchs' Dystrophy [RT-qPCR array CAPH10410]|
This study was performed on CE monolayers with Descemet membrane from patients with late-onset FECD. Microarray expression analysis was performed on 8 samples (n = 4 FECD, n = 4 normal). RT-qPCR was done for 179 genes of interest and at least three reference genes (n = 9 FECD, n = 8 normal). Molecules of interest were validated with immunohistochemical and immunofluorescent staining on fresh corneal endothelial whole-mounts (n = 55 FECD, n = 15 normal). All stainings were optimized on external control tissues, which were included as batch controls. Dye swaps ensured specificity of double staining.
Joost van den Oord