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[…] TCC and grown on tryptic soya agar medium (TSA; HiMedia) at 30 °C. Genomic DNA was extracted from 36 hour old culture using ZR Fungal/Bacterial DNA MiniPrepTM as per manufacturer's instructions. Identification was reconfirmed using 16S rRNA sequencing. Amplification and sequencing of 16S rRNA were performed as described by Mayilraj et al. in 2006 . To determine the phylogenetic relationship of strain MTCC 11364, the 16S rRNA sequence consisting of 1502 bp was compared with those of type strains of species of related genera and identification of phylogenetic neighbors and the calculation of pairwise 16S rRNA gene sequence similarities were achieved using the EzTaxon server and aligned using mega version 5.0 . Phylogenetic trees were constructed using the neighbor-joining algorithm. Bootstrap analysis was performed to assess the confidence limits of the branching ()., The genome of A. junii MTCC 11364 was sequenced using the Illumina-HiSeq 1000 technology. Sequencing resulted in 29,216,732 paired-end reads (insert size of 350 bp) of length 101 bp. A total of 28,584,052 high-quality reads with approximately 810 × coverage were assembled with CLCbio wb6 (word size 40 and bubble size 60) to obtain 248 contigs (N50, 24,956 bp) with 3,549,566 bp and an average G + C content of 38.0%. The functional annotation was carried out by RAST (Rapid Annotation using Subsystem Technology) , shows the subsystem distribution of strain A. junii strain MTCC 11364, tRNA was predicted by tRNAscan-SE 1.23 and rRNA genes by RNAmmer 1.2 . The genome includes 3 rRNA genes (5S, 23S, 16S) and 64 aminoacyl-tRNA synthetase genes., A total of 3294 coding regions (1605 genes transcribed from the positive strand and 1689 from the negative strand) were found in the genome, of which 2332 (71%) could be functionally annotated. The genome coding density is 86% with an average gene length of 907 bp. The annotated genome has 77 genes responsible for resistance to antibiotic and toxic compounds including 10 genes for MDR efflux pumps. One hundred and one genes code for membrane transport proteins. Fifty one genes code for proteins that are involved in oxidative stress response, seven genes for osmotic stress response, fifteen […]

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