Computational protocol: Tumour necrosis factor-α stimulates dehydroepiandrosterone metabolism in human fibroblast-like synoviocytes: a role for nuclear factor-κB and activator protein-1 in the regulation of expression of cytochrome p450 enzyme 7b

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Protocol publication

[…] The promoter sequence of the human Cyp7b gene was identified and exported from the Ensembl database (vs19.34b.2; 9 February 2004) using the MartView export function. As promoter region, -1,000 to +100 nucleotides were selected in relation to the transcription start site. Promoter analysis for transcription factor binding sites was performed using the GEMSLauncher version 3.6 from Genomatrix and MatInspector professional release 7 []. Core and matrix similarity settings were 0.75 and optimized -0.03, respectively. The transcription factor family matrices V$AP1F, V$NFAT, V$NFKB and V$STAT were used. […]

Pipeline specifications

Software tools GenoMatrix, MatInspector
Application GWAS
Organisms Homo sapiens
Chemicals Dehydroepiandrosterone