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Specifications


Application: Gene expression microarray analysis
Number of samples: 11
Release date: Mar 13 2015
Last update date: May 11 2017
Access: Public
Chemicals: Amino Acids
Dataset link Analysis of Klf4 factor stoichiometry effects during iPS cell derivation from mouse embryonic fibroblasts

Experimental Protocol


11 samples were analyzed. MEFs were transfected with one of eight different polycistronic reprogramming vectors or one of two Klf4 variants, followed by FACS sorting for transgene-positive cells. A MEF cell sample, mock transfected with a lacZ expression vector, was used as a reference for all intermediate reprogramming populations. All samples were compared to reveal construct-specific and common gene expression changes.

Repositories


GEO

GSE65468

BioProject

PRJNA274124

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