Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 20
Release date: May 31 2010
Last update date: Mar 21 2016
Access: Public
Taxon: Rattus norvegicus
Dataset link Transcript Profiling of Spinal Dorsal Horn in Response to Electroacupuncture on Rats at 24h

Experimental Protocol


Rats were exposed to different frequencies (2Hz or 100Hz) electroacupuncture stimlation for 30 min and nociceptive testing and returned to home cages for 24 hours before sacrificed. According to the sensitivity of the analgesic response to 2Hz or 100Hz EA stimulation, the rats divided into four groups: 2Hz-HR group, 2Hz-LR group, 100Hz-HR group and 100Hz-LR group. Subsequently analyzed their dorsal horn transcript profile using cDNA microarrays, the rats were without receiving electroacupuncture as control. The tissue of dorsal horn (DH) of the fifth and sixth lumbar (L5 and L6) of four or five rats were selected for transcript analysis in each group. The five control rats were mixed and labeled with cy5, each rat of experiment groups was labeled with cy3.

Repositories


GEO

GSE21733

ArrayExpress

E-GEOD-21733

BioProject

PRJNA129367

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Contact


ke wang