Computational protocol: Involvement of CmWRKY10 in Drought Tolerance of Chrysanthemum through the ABA-Signaling Pathway

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Protocol publication

[…] The amino acid sequence from the previous study [] was used to search for its homolog protein sequences in different dicot species in the NCBI protein database using the BLASTp online tool. All of the sequences with more than 90% coverage were obtained and the sequence alignment of CmWRKY10 was performed along with that of different WRKY orthologs of different species using ClustalX software. For the construction of phylogenetic trees neighbor-joining method with 1000 bootstrap was applied, using MEGA5 software. […]

Pipeline specifications

Software tools BLASTP, Clustal W, MEGA
Databases NCBI Protein
Application Phylogenetics
Organisms Saccharomyces cerevisiae
Diseases Machado-Joseph Disease
Chemicals Abscisic Acid, Oxygen, Superoxides