Computational protocol: Identification and Expression Profiles of IL-8 in Bighead Carp (Aristichthys nobilis) in Response to Microcystin-LR

Similar protocols

Protocol publication

[…] Purified PCR products were ligated into pMD18-T vectors (TaKaRa), and three plasmids were sequenced for each product, respectively. Sequences were analyzed based on nucleotide and protein databases using the BLAST program ( The protein prediction was performed using software at the ExPASy Molecular Biology Server ( The protein family signature was identified by InterPro ( The chemokine CXC domain was defined by National Center for Biotechnology Information (NCBI) conserved-domain database ( Multiple sequence alignment was performed using the CLUSTALW 2 program ( Phylogenetic tree was constructed by using the neighbor-joining (NJ) method with 1000 bootstrap in the Mega 5 software package. […]

Pipeline specifications

Software tools InterPro, Clustal W, MEGA
Databases CDD ExPASy
Applications Phylogenetics, Protein sequence analysis
Organisms Hypophthalmichthys nobilis