Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 8
Release date: Aug 8 2009
Last update date: Mar 21 2012
Access: Public
Chemicals: Glutamic Acid
Dataset link The RpoN Regulon of Escherichia coli O157:H7

Experimental Protocol


Hybridizations measured transcriptional differences between an rpoN null and wild-type (WT) strain of E. coli O157:H7 Sakai at logarithmic and transition phase. Image files (TIFF) of hybridized microarray slides were generated using an Axon 4000B scanner (Molecular Devices), and analyzed using GenePix Pro software (Molecular Devices, ver. 6.0). The resulting microarray intensity data was log2-transformed, and normalized using the LOWESS algorithm in MAANOVA ver. 0.98-8 (R ver. 2.2.1).

Repositories


GEO

GSE17467

ArrayExpress

E-GEOD-17467

BioProject

PRJNA118749

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Contact


James Riordan
James Timothy Riordan