Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 34
Release date: Dec 25 2011
Last update date: Oct 29 2018
Access: Public
Dataset link Programming human pluripotent stem cells into adipocytes [Affymetrix]

Experimental Protocol


Mesenchymal progenitor cells (MPCs) derived from human embryonic stem cells hESCs and induced pluripotent stem cells (iPSCs) along with adipose-derived stromal vascular cells (ADSVCs) were subjected to induction of PPAR2 and compared to primary fat samples. Overall 2 ADSVC (ADSVC 24 nd 49) lines, 1 hESC (HUES9) line and 1 iPSC (BJRiPS) line were differentiated into MPCs, PPAR2 programmed, and compared to untreated MPCs and primary fat samples from 2 individuals. Each condition is either represented in duplicate or triplicate on affymetrix HuGene-1_0-st arrays. MPCs derived from the hESC lines HUES2 and HUES8, ADSVCs, and BJRiPS were also run on a separate platform (HG-U133_Plus_2) with more GEO presence to facilitate analysis (34 samples, two platforms total). Supplementary file(s): GeneSymbol-collapsed data represent the final normalized data used for analyses in the manuscript.

Repositories


GEO

GSE30038

ArrayExpress

E-GEOD-30038

BioProject

PRJNA155147

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Contact


Tim Ahfeldt