Computational protocol: Identification of new reference genes for the normalisation of canine osteoarthritic joint tissue transcripts from microarray data

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Protocol publication

[…] All methods of stability analysis agreed in finding the new genes MRPS7 and MRPS25 to be stably expressed. Likewise, C7orf28B and NCK2 were determined to be the least stably expressed genes by both geNorm (Figure ) and NormFinder (Figure ). GAPDH was identified as the 4th most stably expressed gene by both geNorm and Bestkeeper©, and the 8th most stably expressed gene by NormFinder. […]

Pipeline specifications

Software tools NormFinder, BestKeeper
Application qPCR
Organisms Canis lupus familiaris
Chemicals Glyceraldehyde 3-Phosphate