Computational protocol: Polymethoxyflavone Apigenin-Trimethylether Suppresses LPS-Induced Inflammatory Response in Nontransformed Porcine Intestinal Cell Line IPEC-J2

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Protocol publication

[…] Relative gene expression levels of the genes of interest were calculated by the Relative Expression Software Tool (REST) 2009 Software (Qiagen GmbH, Hilden, Germany). Statistical analysis of other data was performed with STATISTICA 10 software (StatSoft Inc., Tulsa, USA). Differences between means were evaluated by one-way ANOVA, with data of normal distribution, and homogeneity of variances was confirmed. To compare treated groups to controls we used Dunnett post-hoc test. For the comparison of different treatments we used Fisher LSD test. Level of significance was set at P < 0.05. All values were expressed as means ± standard deviations. […]

Pipeline specifications

Software tools REST, Statistica
Applications Miscellaneous, qPCR
Organisms Homo sapiens
Chemicals Flavonoids, Hydrogen Peroxide, Apigenin