Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 2
Release date: Sep 30 2015
Last update date: Feb 4 2016
Access: Public
Dataset link Ovine rumen papillae biopsy via oral endoscopy; a rapid and repeatable method for serial sampling

Experimental Protocol


Four adult Coopworth ewes were fasted for either 4 or 24 hours. Animals were sedated, placed in a dorsally recumbent position at 45 degrees head upright, and endoscope inserted via a tube inserted into the mouth. Biopsies of rumen papillae were taken from the ventral surface of the rumen atrium under visual guidance. Multiple rumen papillae biopsies were collected from several animals. Video of the rumen atrium and reticulum was also collected. The animals recovered uneventfully. Biopsies were subsequently used for extraction and sequencing of mRNA.

Repositories


GEO

GSE68791

ENA

SRP058234

BioProject

PRJNA283849

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