Computational protocol: Transcription factor NFE2L2/NRF2 is a regulator of macroautophagy genes

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Protocol publication

[…] Different band intensities corresponding to immunoblot detection of protein samples were quantified using MCID software (MCID, Cambridge, UK). Fluorescence intensity and M1 and M2 colocalization coefficients were measured by Mander's analysis using the ImageJ JACoP plug-in. Student t test was used to assess differences between groups. P<0.05 was considered significant. Unless otherwise indicated, all experiments were performed at least 3 times and all data presented in the graphs are the mean of at least 3 independent samples. Results are expressed as mean ± SEM. […]

Pipeline specifications

Software tools ImageJ, JACoP
Application Microscopic phenotype analysis
Organisms Mus musculus, Homo sapiens, Caenorhabditis elegans, Lentivirus
Diseases Alzheimer Disease
Chemicals Hydrogen Peroxide