Computational protocol: Selection of Reference Genes for Expression Analysis Using Quantitative Real-Time PCR in the Pea Aphid, Acyrthosiphon pisum (Harris) (Hemiptera, Aphidiae)

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Protocol publication

[…] All biological replicates were used to calculate the average Ct value. The stability of the ten housekeeping genes were evaluated by algorithms geNorm , NormFinder , BestKeeper , and the comparative ΔCt method . Finally, we compared and ranked the tested candidates based on a web-based analysis tool RefFinder (http://www.leonxie.com/referencegene.php). […]

Pipeline specifications

Software tools NormFinder, BestKeeper
Application qPCR
Organisms Acyrthosiphon pisum
Chemicals NAD, Succinic Acid