Computational protocol: Analysis of the N-terminal region of human MLKL, as well as two distinct MLKL isoforms, reveals new insights into necroptotic cell death

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Protocol publication

[…] The model of full-length MLKL1 was generated using Modeller [] with the structures PDB: 2MSV [], PDB: 4MWI [] and PDB: 4BTF [] as templates. The MLKL2 model was generated using the I-TASSER modelling server []. All images were prepared using PyMOL (DeLano Scientific LLC). [...] Phosphorylation sites were predicted using the online prediction servers NetPhos 2.0 [] and Predikin []. Predictions with Predikin were made for both MLKL isoforms as RIP3 kinase substrates. […]

Pipeline specifications

Software tools MODELLER, I-TASSER, PyMOL, NetPhos, Predikin
Applications PTM analysis, Protein structure analysis
Organisms Homo sapiens
Chemicals Amino Acids