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easy-dhpsf specifications


Unique identifier OMICS_28425
Name easy-dhpsf
Alternative name easy-double-helix point spread function
Software type Application/Script
Interface Graphical user interface
Restrictions to use None
Operating system Windows
Programming languages MATLAB
License BSD 3-clause “New” or “Revised” License
Computer skills Medium
Version 1.02
Stability Stable
Maintained Yes




No version available



  • person_outline Matthew Lew <>

Publication for easy-double-helix point spread function

easy-dhpsf citations


3D single molecule super resolution microscopy with a tilted light sheet

PMCID: 5760554
PMID: 29317629
DOI: 10.1038/s41467-017-02563-4

[…] easy-pupil-finder software (https://sourceforge.net/projects/easy-pupil-finder/). calibration and fitting analysis of dh-psf images was performed using a modified version of the open-source easy-dhpsf software (https://sourceforge.net/projects/easy-dhpsf/)., the single-molecule localizations generated and analyzed during the current study are available from the corresponding author […]


Generalized recovery algorithm for 3D super resolution microscopy using rotating point spread functions

PMCID: 4973222
PMID: 27488312
DOI: 10.1038/srep30826
call_split See protocol

[…] and biplane methods. however, psfs recorded in these types of 3d microscopies can be well-fit by gaussians or elliptical gaussians, which is usually not possible for phase mask engineered psfs. easy-dhpsf is a useful algorithm to analyze 3d single-particle tracking data using a double-helix phase mask, but it requires the recorded psfs to have no overlap, which, as we discuss below, […]

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easy-dhpsf institution(s)
Department of Chemistry, Stanford University, Stanford, CA, USA; Department of Electrical Engineering, Stanford University, Stanford, CA, USA
easy-dhpsf funding source(s)
Supported by a National Science Foundation Graduate Research Fellowship, 3Com Corporation Stanford Graduate Fellowship and a National Institute of General Medical Sciences Grant R01GM085437.

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