easy-FRAP specifications

Information


Unique identifier OMICS_25623
Name easy-FRAP
Alternative names easyFRAP, easyFRAP-web
Software type Toolkit/Suite
Interface Graphical user interface
Restrictions to use None
Input data The intensity measurements from the bleached area, the total fluorescence area, a background area and the corresponding time-points.
Input format CSV,TXT,XLS
Output format CSV,TXT,XLS
Operating system Mac OS, Windows
Programming languages MATLAB
License GNU General Public License version 3.0
Computer skills Medium
Stability Stable
Maintained Yes

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Documentation


Maintainers


  • person_outline Zoi Lygerou <>
  • person_outline Maria Rapsomaniki <>

Additional information


http://ccl.med.upatras.gr/data/uploads/easyfrap_quick_guide.pdf

Publications for easy-FRAP

easy-FRAP in publications

 (2)
PMCID: 5596426
PMID: 28622300
DOI: 10.1038/cdd.2017.88

[…] regions of interest: roi1: bleached area, roi2: unbleached area within the cell and roi3: background. normalized frap recovery curves and the mobile fraction were calculated using the program easy frap., to analyze the dynamics of the rfp-gfp-lc3 fusion protein, gfp and rfp channels were acquired every minute for up to 4 h using the imaging system described above. the extent […]

PMCID: 4611717
PMID: 24922074
DOI: 10.1038/cddis.2014.243

[…] of interest (rois): roi1: bleached area; roi2: unbleached area within the cell; and roi3: background. normalized frap recovery curves and the mobile fractions were calculated using the program easy frap., to analyze the dynamics of the rfp–gfp–lc3 fusion protein, gfp and rfp channels were acquired every min for up to 4 h using the imaging system described above. the extent of autophagosome […]


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easy-FRAP institution(s)
Laboratory of Biology School of Medicine, University of Patras, Patras, Greece; Laboratory of Physiology, School of Medicine, University of Patras, Patras, Greece
easy-FRAP funding source(s)
Supported by a grant from the Association for International Cancer Research.

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