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EasyqpcR specifications


Unique identifier OMICS_02313
Name EasyqpcR
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
Programming languages R
License GNU General Public License version 2.0
Computer skills Advanced
Version 1.21.0
Stability Stable
plyr, knitr, plotrix, matrixStats, gWidgetsRGtk2, SLqPCR, qpcrNorm, qpcR
Maintained Yes


No version available


EasyqpcR citations


The ripening disorder berry shrivel affects anthocyanin biosynthesis and sugar metabolism in Zweigelt grape berries

PMCID: 5778156
PMID: 29075874
DOI: 10.1007/s00425-017-2795-4
call_split See protocol

[…] qPCR expression analyses were calculated as normalized relative quantities (NRQ) with the R package easyqPCR (Le Pape ). All statistical comparisons were conducted using IBM SPSS Statistics 21. Normal distribution of data sets was tested using the Shapiro–Wilk test. Outliers were determined accordin […]


Small secreted proteins from the necrotrophic conifer pathogen Heterobasidion annosum s.l. (HaSSPs) induce cell death in Nicotiana benthamiana

Sci Rep
PMCID: 5554239
PMID: 28801666
DOI: 10.1038/s41598-017-08010-0

[…] sec, 60 °C for 10 sec, and 72 °C for 10 sec). Following cycle completion, samples were subjected to melting curve analysis to assess the primer specificity. The qPCR data were analysed in R using the EasyqpcR package and normalized using 2 reference genes (EF1a and Actin). Primer information is summarized in Supplementary Table . […]


Albugo imposed changes to tryptophan derived antimicrobial metabolite biosynthesis may contribute to suppression of non host resistance to Phytophthora infestans in Arabidopsis thaliana

BMC Biol
PMCID: 5358052
PMID: 28320402
DOI: 10.1186/s12915-017-0360-z

[…] ation were calculated from three replicate reaction wells. The cycle threshold values and primer efficiencies were then used to calculate normalized relative quantities (NRQs) for each gene using the EasyqpcR package [] in R. NRQs were then log2 transformed [] and statistical analyses performed as described below. […]


Conserved Epigenetic Mechanisms Could Play a Key Role in Regulation of Photosynthesis and Development Related Genes during Needle Development of Pinus radiata

PLoS One
PMCID: 4429063
PMID: 25965766
DOI: 10.1371/journal.pone.0126405

[…] level of p< 0.05 was considered significant for all statistical analyses. Five biological replicates were analysed. Quantitative PCR data was processed according to Hellemans et al. [] and R packages EasyqPCR and SLqPCR before applying ANOVA. […]


Activation of defence pathways in Scots pine bark after feeding by pine weevil (Hylobius abietis)

BMC Genomics
PMCID: 4422480
PMID: 25943104
DOI: 10.1186/s12864-015-1546-9
call_split See protocol

[…] 2). Two reference genes, elongation factor 1 alfa (EF1-α, []) and alfa tubulin (TUBA []), were used to normalise the data. Finally, the Cp values were imported and analysed in R software [] using the EasyqpcR package []. […]


A survey of tools for the analysis of quantitative PCR (qPCR) data

PMCID: 5129434
PMID: 27920994
DOI: 10.1016/j.bdq.2014.08.002

[…] plots. dpcR uses the shiny R packages to provide an interactive web or off-line graphical user interface application, which can be used by both R novices and experts.The recently released R package EasyqpcR is based on the qBase algorithms and calculates relative quantities using Cq values. It is able to determine amplification efficiencies using dilution series, and calculates normalization fa […]


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