EasyqpcR statistics

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EasyqpcR specifications

Information


Unique identifier OMICS_02313
Name EasyqpcR
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
Programming languages R
License GNU General Public License version 2.0
Computer skills Advanced
Version 1.21.0
Stability Stable
Requirements
plyr, knitr, plotrix, matrixStats, gWidgetsRGtk2, SLqPCR, qpcrNorm, qpcR
Maintained Yes

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Documentation


EasyqpcR in publications

 (6)
PMCID: 5778156
PMID: 29075874
DOI: 10.1007/s00425-017-2795-4

[…] offenburg, germany) by the slope linear regression function. results are expressed as nmol/min., qpcr expression analyses were calculated as normalized relative quantities (nrq) with the r package easyqpcr (le pape ). all statistical comparisons were conducted using ibm spss statistics 21. normal distribution of data sets was tested using the shapiro–wilk test. outliers were determined […]

PMCID: 5554239
PMID: 28801666
DOI: 10.1038/s41598-017-08010-0

[…] 60 °c for 10 sec, and 72 °c for 10 sec). following cycle completion, samples were subjected to melting curve analysis to assess the primer specificity. the qpcr data were analysed in r using the easyqpcr package and normalized using 2 reference genes (ef1a and actin). primer information is summarized in supplementary table ., , electronic supplementary material , supplementary […]

PMCID: 5358052
PMID: 28320402
DOI: 10.1186/s12915-017-0360-z

[…] were calculated from three replicate reaction wells. the cycle threshold values and primer efficiencies were then used to calculate normalized relative quantities (nrqs) for each gene using the easyqpcr package [] in r. nrqs were then log2 transformed [] and statistical analyses performed as described below., plants were sprayed with albugo or water, and subsequently inoculated with p. […]

PMCID: 4429063
PMID: 25965766
DOI: 10.1371/journal.pone.0126405

[…] of p< 0.05 was considered significant for all statistical analyses. five biological replicates were analysed. quantitative pcr data was processed according to hellemans et al. [] and r packages easyqpcr and slqpcr before applying anova., to assess how epigenetic mechanisms might regulate needle development and maturation in pinus radiata, we sampled needles at different developmental stages […]

PMCID: 4422480
PMID: 25943104
DOI: 10.1186/s12864-015-1546-9

[…] two reference genes, elongation factor 1 alfa (ef1-α, []) and alfa tubulin (tuba []), were used to normalise the data. finally, the cp values were imported and analysed in r software [] using the easyqpcr package []., the data set supporting the results of this article is included within the article and its additional files., additional file 1: table s1. additional file 2: table s2. , […]


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