Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 8
Release date: Aug 1 2013
Last update date: Aug 9 2018
Access: Public
Taxon: Homo sapiens, Mus musculus
Dataset link Location of indigenous human HSCs reveals functional properties of HSCs that are dictated by anatomic and cellular architecture of bone marrow I

Experimental Protocol


Lin- MNCs from human Umbilical Cord blood (CB) were injected via tail vein into sublethally irradiated NOD/SCID adult mice. After 10 weeks post-transplantation, engrafted-CB cells were sorted based on the co-expression of CD45 and CD34, and absence or presence of CD38 using a FACSAria II (BD). Total RNA from purified populations was extracted and amplified as described previously (Shojaei et al., 2005). Amplified-labeled RNA was hybridized to HG-U133Plus v2.0 chip.

Repositories


GEO

GSE47934

ArrayExpress

E-GEOD-47934

BioProject

PRJNA208389

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Contact


Mickie Bhatia