Dataset features

Specifications


Dataset type: Expression profiling by high throughput sequencing, Other
Number of samples: 7
Release date: Dec 20 2018
Last update date: Dec 27 2018
Access: Public
Dataset link TSS identification of Rap1-regulated transcripts by 5' end RNA sequencing

Experimental Protocol


5’ end RNA sequencing analysis of Saccharomyces cerevisiae S288C strains transformed with Os TIR1 ligase and auxin-inducible degron (IAA7) tagged Rap1, or wild-type control strains. 3 biological replicates of each condition are analysed. For each biological replicate, cells were grown overnight in YPD medium, diluted, and treated with 500 μM 3-indole-acetid acid (3-IAA, auxin) for RAP1_AID_IAA samples or left untreated for WT samples. RAP1_AID_IAA samples were taken 2 hours after treatment, and for WT control samples were taken in mid-logarithmic growth. Details for library preparation are described below.

Repositories


GEO

GSE110000

ENA

SRP132072

BioProject

PRJNA432634

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Contact


Andrew Wu