Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 18
Release date: Mar 31 2018
Last update date: Sep 27 2018
Access: Public
Diseases: Lung Diseases, Lung Neoplasms, Neoplasms, Carcinoma, Lewis Lung
Dataset link Expression profile comparison between paired tumor- and normal tissue-associated MSCs from lung carcinoma patients

Experimental Protocol


MSCs were isolated from paired lung tumor (T-) and normal tissues (N-) from 9 patients diagnosed with lung carcinoma and characterized for surface expression markers and differentiation potential. RNA was extracted at culture passage 5 or 6 from fresh MSCs (frozen in DMSO-FBS10%) to be sure to have pure MSC cell culture representative of the pathophysiological characteristics of the primary samples. Paired N- and T-MSC expression profiles were analyzed using Affymetrix microarrays. We sought to identify differentially expressed genes between N- and T-MSCs to gain insight into the mechanisms whereby the stroma promotes lung cancer metastasis.

Repositories


GEO

GSE104636

BioProject

PRJNA413379

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Contact


Mathieu Quinodoz

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