Dataset features

Specifications


Application: DNA methylation array analysis
Number of samples: 131
Release date: Oct 30 2015
Last update date: Jul 25 2018
Access: Public
Diseases: Down Syndrome
Chemicals: 5-Methylcytosine
Dataset link Trans-effects of chromosome aneuploidies on DNA methylation patterns: DNA methylation analysis of Down syndrome in human brain tissues and cells

Experimental Protocol


Bisulfite converted DNA from 119 samples from Down syndrome patients and controls were hybridised to the Illumina Infinium 450k Human Methylation Beadchip. In addition, we re-analyzed 6 Down syndrome and 6 control cerebellum DNA samples on the 450K BeadChips using an adaptation of the Illumina probe preparation protocol (TrueMethyl kit; Cambridge Epigenetics, CEGX), in which parallel analyses of bisulfite and oxidative bisulfite DNA for each sample allows assessment of the relative contributions of 5mC and 5hmC to net methylation.

Repositories


GEO

GSE74486

ArrayExpress

E-GEOD-74486

BioProject

PRJNA300574

Download


Contact


Benjamin Tycko

Dataset Statistics

info

Citations per year

Dataset publication