In silico resources developed to facilitate scientific community fighting against deadly virus EBOLA. EbolaVCR enlists possible vaccine candidates, therapeutic siRNAs and other related resources. Firstly, we predicted and identified experimentally validated epitopes in each of the antigens/proteins of the five known ebolaviruses. Secondly, we generated all the possible overlapping 9mer peptides from the proteins of ebolaviruses. Thirdly, conserved peptides across all the five ebolaviruses (four human pathogenic species) with no identical sequence in the human proteome, based on 1000 Genomes project, were identified. Finally, we identified peptide or epitope-based vaccine candidates that could activate both the B- and T-cell arms of the immune system. In addition, we also identified efficacious siRNAs against the mRNA transcriptome (absent in human transcriptome) of all the five ebolaviruses.
Bioinformatics Centre, CSIR-Institute of Microbial Technology, Chandigarh, India; CSIR-Institute of Genomics and Integrative Biology, Mathura Road, New Delhi, India
EbolaVCR funding source(s)
This work was supported by the Council of Scientific and Industrial Research through the OSDD consortium and GENESIS projects; the Department of Biotechnology, Government of India for funding the project through BTISNET; the Indian Council of Medical Research; the J. C. Bose National Fellowship conferred by the Department of Science and Technology.