Computational protocol: Endothelial Progenitor Cell Migration-Enhancing Factors in the Secretome of Placental-Derived Mesenchymal Stem Cells

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[…] Mass spectrometry analysis was performed at the National Center for Genetic Engineering and Biotechnology (BIOTEC), Thailand. The proteins presented in each PL-MSCs conditioned medium fraction were digested by incubation with trypsin and analyzed by ESI ion trap mass spectrometry. Identification and quantification of each protein was determined by DeCyder MS differential analysis software 2.0 (GE Healthcare, USA) and MASCOT search engine software (Matrix Science, UK) based on NCBInr human protein databases. The identified proteins were then categorized into nonsecretory, classical secretory, and nonclassical secretory proteins by SignalP and SecretomeP software (Center for Biological Sequence Analysis (CBS), Denmark). Finally, the secretory proteins were further categorized by PANTHER and UniProt software into separated groups according to their functions. […]

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