Dataset features

Specifications


Application: RNA-seq analysis, sRNA-seq analysis
Number of samples: 4
Release date: Jan 1 2014
Last update date: Nov 17 2017
Access: Public
Dataset link Ago1 vs Ago2-IP small RNA deep-sequencing with age in Drosophila

Experimental Protocol


3d and 30d FLAG-HA-Ago2 male flies were collected. Ago1 and Ago2 were immunoprecipitated by anti-Ago1 and anti-FLAG M2 beads respectively. RNA was purified from the beads, P32-labeled, and small RNA fraction was gel-purififed. Small RNA libraries were prepared using Illumina's TruSeq small RNA sample preparation kit (#RS-200-0012, Illumina, Inc. San Diego, CA), following the manufacturer's protocol. The libraries were multiplexed and sequenced on HiSeq2000 platform (Illumina).

Repositories


GEO

GSE52961

ArrayExpress

E-GEOD-52961

ENA

SRP033489

BioProject

PRJNA230555

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Contact


Ammar Naqvi