|Application:||RNA-seq analysis, sRNA-seq analysis|
|Number of samples:||4|
|Release date:||Jan 1 2014|
|Last update date:||Nov 17 2017|
|Dataset link||Ago1 vs Ago2-IP small RNA deep-sequencing with age in Drosophila|
3d and 30d FLAG-HA-Ago2 male flies were collected. Ago1 and Ago2 were immunoprecipitated by anti-Ago1 and anti-FLAG M2 beads respectively. RNA was purified from the beads, P32-labeled, and small RNA fraction was gel-purififed. Small RNA libraries were prepared using Illumina's TruSeq small RNA sample preparation kit (#RS-200-0012, Illumina, Inc. San Diego, CA), following the manufacturer's protocol. The libraries were multiplexed and sequenced on HiSeq2000 platform (Illumina).