|Application:||Peptide array analysis|
|Number of samples:||6|
|Release date:||Aug 25 2017|
|Last update date:||Jan 24 2018|
|Dataset link||Therapeutic antibodies to ganglioside GD2 evolved from highly selective germline antibodies I|
Germline variable regions of 3F8 and ch14.18 were determined. Germline and affinity mature antibody DNA were synthesized and transfected into HEK293 cell lines. Secreted antibodies were purified from supernatant using affinity chromatography. Antibodies were evaluated on multiple assay platforms, including glycan microarrays, protein microarrays, and cell binding assays.