Computational protocol: Phosphorylated Nucleolin Interacts with Translationally Controlled Tumor Protein during Mitosis and with Oct4 during Interphase in ES Cells

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Protocol publication

[…] Colocalization was visualized using ImageJ (http://rsb.info.nih.gov/ij). Confocal micrographs were collected at 0.38 µM intervals to create z-axis stacks, and images rendered from the z-axis stacks were analyzed with the BioPix iQ 2.0 software. Mitotic and interphase cells were selected manually. A minimum of five different z-axis stacks, containing at least 10 cells each, were taken for each analysis. Data are represented as mean±SEM (standard error of mean). SPSS 13.0 was used for statistical evaluation of colocalization results generated by BioPix iQ 2.0. One-way analysis of variance followed by post hoc and Turkey's test, were done to evaluate significant changes between groups. Values are considered significant if p ≤ 0.01 for the differences between analyzed groups. […]

Pipeline specifications

Software tools ImageJ, SPSS
Applications Miscellaneous, Microscopic phenotype analysis
Organisms Mus musculus, Homo sapiens
Diseases Neoplasms
Chemicals Tretinoin