Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 7
Release date: Apr 27 2015
Last update date: Jul 19 2017
Access: Public
Diseases: Brain Ischemia, Myositis, Neoplasms, Neoplasms, Germ Cell and Embryonal
Dataset link Knockout of Vdac1 activates the HIF signaling pathway and induces tumor development in vivo by promoting vascular remodeling and inflammation

Experimental Protocol


Profiling of VDAC1-/- and wild-type (Wt) Mouse Embryonic Fibroblasts (MEFs) was performed using whole genome mouse microarrays. in normoxic or hypoxic conditions. MEF (Wt and Vdac1-/-) were incubated in normoxia (Nx, ie: 20% O2) or hypoxia (Hx, ie: 1% O2) for 72h and then lysed prior to RNA isolation, labelling and hybridization on microarrays. One color experiment with 2 biological replicates (marked 1 or 2) of the 4 experimental conditions. One condition (MEF WT Nx2) has been removed from the statistical analysis after microarray quality check due to high background. Total of 7 samples.

Repositories


GEO

GSE63247

ArrayExpress

E-GEOD-63247

BioProject

PRJNA267104

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Contact


Kevin Lebrigand