Describes several key properties of iJO1366, an E. coli genome-scale reconstruction. ECC2 contains 499 reactions (thereof 198 reversible) and 486 internal as well as 33 external metabolites. It yields a maximal biomass above 99.9% of the genome-scale maximum for all substrates. The model can be used to find intervention strategies leading to desired phenotypes in the core model. It can be extended by further knockouts to a valid minimal cut sets (MCS) for E. coli genome-scale (ECGS).