|Number of samples:||1|
|Release date:||Aug 1 2018|
|Last update date:||Sep 6 2018|
|Dataset link||Comparative transcriptomic analyses and single-cell RNA sequencing of the freshwater planarian Schmidtea mediterranea identifies major cell types and pathway conservation|
Wildtype animals were mechanically dissociated in Calcium-magnesium free media (CMF) with 1% BSA and stained with calcein to label live cells. Calcein-positive cells were collected by FACS. Cells were barcoded and cDNA libraries were prepared in nanoliter droplets using Drop-seq. The cDNA library pool was sequenced to a total depth of 480 million paired-end reads on an Illumina NextSeq500.