Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 1
Release date: Aug 1 2018
Last update date: Sep 6 2018
Access: Public
Chemicals: Nucleotides
Dataset link Comparative transcriptomic analyses and single-cell RNA sequencing of the freshwater planarian Schmidtea mediterranea identifies major cell types and pathway conservation

Experimental Protocol


Wildtype animals were mechanically dissociated in Calcium-magnesium free media (CMF) with 1% BSA and stained with calcein to label live cells. Calcein-positive cells were collected by FACS. Cells were barcoded and cDNA libraries were prepared in nanoliter droplets using Drop-seq. The cDNA library pool was sequenced to a total depth of 480 million paired-end reads on an Illumina NextSeq500.

Repositories


GEO

GSE115280

ENA

SRP149686

BioProject

PRJNA474473

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Contact


Bret Pearson