Dataset features

Specifications


Dataset type: Other
Number of samples: 647
Release date: May 1 2018
Last update date: Nov 20 2018
Access: Public
Taxon: Homo sapiens
Dataset link Kinetics and fidelity of the repair of Cas9-induced double-strand DNA breaks

Experimental Protocol


We transiently transfected K562#17 with a plasmid encoding a sgRNA. Twenty-four hours after transfection we stabilized Cas9 by adding Shield-1. We collected cells at various time points, isolated genomic DNA, amplified a ~300-bp region around the sgRNA target site by PCR and subjected the products to high-throughput sequencing to determine the intact and indel fractions. 8 replicates for sgRNA-LBR2, 6 replicates for sgRNA-AAVS1, 6 replicates sgRNA-LBR8, 5 replicates chr11, 2 replicates sgRNA LBR2/LBR8 combined. Double cut t=60 for sgRNA LBR2/LBR5, sgRNA LBR2/LBR6, sgRNA LBR2/LBR7, sgRNA LBR2/LBR8, sgRNA LBR2/LBR9

Repositories


GEO

GSE113129

ENA

SRP140510

BioProject

PRJNA450382

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Contact


Bas van Steensel