Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Nov 15 2013
Last update date: Mar 10 2014
Access: Public
Chemicals: Sirolimus
Dataset link Expression analysis of wild-type and transcription factor mutants in response to rapamycin

Experimental Protocol


Four separate wild-type cultures of yeast were grown, and each culture was split into two. Then, one was treated with DMSO and the other one was treated with rapamycin. For transcription factor mutants, two separate cultures were grown, and rapamycin was treated in the same way as treated to the wild type. Total RNA was recovered from each culture, then labeled oligo was prepared following NimbleGene standard protocol. Each chip measures the expression levels of 5,777 genes from yeast.

Repositories


GEO

GSE51376

ArrayExpress

E-GEOD-51376

BioProject

PRJNA222849

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Contact


Vishy Iyer

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