Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 4
Release date: Oct 1 2013
Last update date: May 13 2016
Access: Public
Dataset link Next-Generation Sequencing for Whole Transcriptome Analysis in Scrambled Control and EGFR Knockdown Cells Cultured under Normoxia and Hypoxia

Experimental Protocol


RNA profiles of HeLa cells expressing scrambled control (S) or EGFR shRNA-E1 (A1) that cultured under normoxia or hypoxia (1% O2) for 24h were generated by AB SOLiD curstomarized next-generation sequencing, including both small RNA application and whole transcriptome analysis. S: HeLa expressing scrambled control cultured under normoxia; A1: HeLa expressing EGFR shRNA-E1 cultured under normoxia; HS: HeLa expressing scrambled control cultured under hypoxia for 24h; HA1: HeLa expressing EGFR shRNA-E1 cultured under hypoxia for 24h. In total, 4 biological samples with no replicates resulted in 4 whole transcriptome RNA profiles.

Repositories


GEO

GSE44801

ArrayExpress

E-GEOD-44801

ENA

SRP019229

BioProject

PRJNA191817

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Contact


Mien-Chie Hung