Computational protocol: Bacterial attachment and biofilm formation on surfaces are reduced by small-diameter nanoscale pores: how small is small enough?

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Protocol publication

[…] The total biomass and surface coverage were quantified using COMSTAT, a computer programme designed specifically for this purpose. A threshold value of 3 was assigned to all the individual image stacks. Quantified parameters were: biomass accumulation (μm3/μm2), obtained by dividing the overall biomass volume by the substratum area; and layer coverage, given by the percentage of the area occupied by bacteria in each optical layer. [...] One-way analysis of variance and post-analysis-of-variance Tukey’s test were used to compare multiple means. All analyses were performed with JMP Pro 11 (SAS Institute, Cary, NJ, USA). The adjusted XDLVO model was constructed and computed using Mathematica 9.0 (Wolfram, Champaign, IL, USA). […]

Pipeline specifications

Software tools Comstat, JMP Pro
Applications Miscellaneous, Laser scanning microscopy
Organisms Homo sapiens, Escherichia coli, Listeria monocytogenes, Staphylococcus aureus
Diseases Foodborne Diseases, Staphylococcal Infections
Chemicals Aluminum Oxide