Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Feb 13 2014
Last update date: Aug 9 2018
Access: Public
Diseases: Breast Neoplasms, Neoplasms
Chemicals: Puromycin
Dataset link Nuclear factor kappa B activation-induced anti-apoptosis renders HER2 positive cells drug resistant and accelerates tumor growth

Experimental Protocol


We sorted SKBR-3 cells by fluorescence-activated cell sorting (FACS) to enriched for cell population with elevated HER2 expression, which we termed SKR6. The following cell lines were then created from SKR6 cells: SKR6CA: SKR6 cells retrovirally transduced with constitutively active NF-κB relA/p65 (CAp65), SKR6 vector: SKR6 cells transduced with the pQCXIP empty retroviral vector and selected with puromycin, and SKR6LR: SKR6 cells treated with increasing lapatinib concentrations (0.2 to 5 μM) for several months.

Repositories


GEO

GSE52707

ArrayExpress

E-GEOD-52707

BioProject

PRJNA229869

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Contact


Shannon Bailey
Shannon Terrell Bailey