Foxd1 is required for terminal differentiation of anterior hypothalamic neuronal subtypes

Although the hypothalamus functions as a master homeostat for many behaviors, little is known about the transcriptional networks that control its development. To investigate this question, we analyzed mice deficient for the Forkhead domain transcription factor Foxd1. Foxd1 is selectively expressed in neuroepithelial cells of the prethalamus and hypothalamus prior to the onset of neurogenesis, and is later restricted to neural progenitors of the prethalamus and anterior hypothalamus. During early stages of neurogenesis, we observed that Foxd1-deficient mice showed reduced expression of Six3 and Vax1 in anterior hypothalamus, but overall patterning of the prethalamus and hypothalamus is unaffected. After neurogenesis is complete, however, a progressive reduction and eventual loss of expression of molecular markers of the suprachiasmatic, paraventricular and periventricular hypothalamic is observed. These findings demonstrate that Foxd1 acts in hypothalamic progenitors to allow sustained expression of a subset of genes selectively expressed in mature neurons of the anterior hypothalamus.

Dataset features

Information

Specifications

Application:	RNA-seq analysis
Number of samples:	8
Release date:	May 30 2018
Last update date:	Nov 30 2018
Access:	Public
Diseases:	Hypothalamic Diseases, Hypothalamic Neoplasms
Dataset link	Foxd1 is required for terminal differentiation of anterior hypothalamic neuronal subtypes

Experimental Protocol

We measured gene expression profiles of wild-type and Foxd1-mutant hypothalamic tissues separately at E12.0, E12.5 and E17.5

Repositories

GEO

GSE108400

ENA

SRP127333

BioProject

PRJNA427132

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Contact

Jie Wang