Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 18
Release date: Apr 18 2016
Last update date: Jun 21 2017
Access: Public
Chemicals: Sucrose
Dataset link MicroRNAs control mRNA fate by compartmentalizing mRNAs based on 3’UTR length

Experimental Protocol


Pachytene spermatocytes, round and elongating/elongated spermatids were purified from adult mouse testes using the STA-PUT method. We fractionated the purified spermatogenic cells into RNP, monoribosome, and polyribosome fractions using a continuous sucrose gradient ultracentrifugation method, as described.

Repositories


GEO

GSE80353

ENA

SRP073385

BioProject

PRJNA318649

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Contact


Wei Yan