|Number of samples:||18|
|Release date:||Apr 18 2016|
|Last update date:||Jun 21 2017|
|Dataset link||MicroRNAs control mRNA fate by compartmentalizing mRNAs based on 3’UTR length|
Pachytene spermatocytes, round and elongating/elongated spermatids were purified from adult mouse testes using the STA-PUT method. We fractionated the purified spermatogenic cells into RNP, monoribosome, and polyribosome fractions using a continuous sucrose gradient ultracentrifugation method, as described.