Dataset features

Specifications


Application: BS-seq analysis
Number of samples: 7
Release date: Aug 17 2012
Last update date: Jun 21 2017
Access: Public
Chemicals: Biotin, Cytosine, Thymine
Dataset link Genome-wide distribution of 5-formylcytosine in ES cells is associated with transcription and depends on TDG

Experimental Protocol


We devised a method to map 5-formylcytosine (5fC) by linking a biotin tag to 5fC for pulldown and high-throughput sequencing. We mapped 5fC in the following samples of mouse embryonic stem cells (J1): Wild-type ES cells (two replicates); ES cells transfected with siRNA targeting TDG (two replicates); ES cells transfected with non-targeting siRNA (two replicates). One genomic input library was also sequenced to detect and correct biases in fragment enrichment.

Repositories


GEO

GSE40148

ArrayExpress

E-GEOD-40148

ENA

SRP014870

BioProject

PRJNA172927

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Contact


Dario Beraldi

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