|Number of samples:||7|
|Release date:||Aug 17 2012|
|Last update date:||Jun 21 2017|
|Chemicals:||Biotin, Cytosine, Thymine|
|Dataset link||Genome-wide distribution of 5-formylcytosine in ES cells is associated with transcription and depends on TDG|
We devised a method to map 5-formylcytosine (5fC) by linking a biotin tag to 5fC for pulldown and high-throughput sequencing. We mapped 5fC in the following samples of mouse embryonic stem cells (J1): Wild-type ES cells (two replicates); ES cells transfected with siRNA targeting TDG (two replicates); ES cells transfected with non-targeting siRNA (two replicates). One genomic input library was also sequenced to detect and correct biases in fragment enrichment.