Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Dec 30 2017
Last update date: Jan 19 2018
Access: Public
Taxon: Mus musculus
Dataset link Identification of a myofibroblast-specific expression signature in skin wounds

Experimental Protocol


Non-hematopoietic (CD45-)/non-endothelial (CD31-) cell populations with low expression of SMA-GFP from dermis (SMA-GFP+) and with high expression from wounds 7 days post injury (SMA-GFP+++) were isolated by cell sorting from transgenic SMA-GFP mice. Total RNA of was isolated with TRIZOL (Life Technologies) and RNA quality was confirmed using micro capillary electrophoresis (2100 Bioanalyzer, Agilent). ~50ng of RNA was amplified, labeled and hybridized to Sureprint G3 human GE 8x60K or whole genome mRNA microarray according to the manufacturer’s specifications (Low input Quick Amp Labeling kit, Agilent). The arrays were scanned (Agilent G2595C scanner), data extracted and processed to create a generic gene level experiments from two technologies using the Genespring 14.5 software (Agilent).

Repositories


GEO

GSE95786

BioProject

PRJNA378407

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Contact


Bent Brachvogel