Dataset features

Specifications


Application: ChIP-seq analysis
Number of samples: 22
Release date: Feb 18 2011
Last update date: Apr 20 2015
Access: Public
Dataset link Multiplexed identification of the genomic targets of DNA-binding proteins

Experimental Protocol


These data contain Ty5 insertion sites mapped by an Illumina GAII analyzer in the S. cerevisiae genome for the background strain without any Sir4 present (1 run), in strains expressing Sir4-tagged copies of three well-characterized TFs: Gal4, Leu3, and Gcn4 (1 run each), and a multiplex of eight Sir4-tagged TFs pooled in a single experiment (2 biological replicates), and insertions from the Thi2-Sir4 fusion expressed from its native locus in two conditions (1 run each). The format of each insertions file is [chromosome number] [position of genomic base] [direction of insertion] [number of reads at that position]. Raw sequencing data comes in two varieties. Paired-end data contains a 5 bp barcode at the beginning of read #2. Single-end data contains a 2 bp barcode on the beggining of read #1.

Repositories


GEO

GSE27381

ArrayExpress

E-GEOD-27381

ENA

SRP005862

BioProject

PRJNA137021

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Contact


David Mayhew