Computational protocol: Activity‐dependent redistribution of Kv2.1 ion channels on rat spinal motoneurons

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Protocol publication

[…] Microscope images were prepared by adjusting contrast and brightness in Image Pro Plus Software (Media Cybernetics, Bethesda, MD) and always preserved all the information content of the images. Figures were composed, using CorelDraw (v. 12.0). Graphs were composed in SigmaPlot (version 9.0, Systat Software, SPSS Inc, Chicago, IL). Some images were sharpened using a “high gauss” filter in image pro. Quantification was always carried out in original unprocessed images. [...] All statistical tests were performed in SigmaPlot (version 9.0, Systat Software, SPSS Inc) software. Nonparametric Mann–Whitney Wilcoxon rank sum tests were used in all pairwise comparisons. Statistical significance was set at P = 0.05 and data presented in figures is mean ± SD. […]

Pipeline specifications

Software tools CorelDraw, SigmaPlot, SPSS
Application Miscellaneous
Organisms Rattus norvegicus
Diseases Peripheral Nervous System Diseases
Chemicals Glutamic Acid